RNA

Part:BBa_K3081105:Design

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)


A meaningless sequence with two flanking BsaI sites,facilitating replacement of intended sgRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 406
    Illegal BsaI.rc site found at 2


Design Notes

No


Source

E. coli

References